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RAP80 foci in cells treated with SUMO4 siRNA, expressing 6×His-HA-SUMO4-WT or 6×His-HA-SUMO4-QFI-A irradiated (4 Gy), and fixed 2 h later. Images, left, scale bar, 10 μm. N∼ 150 cells per condition from three experiments. Bars are mean ± SEM. Statistical analysis: two-tailed t test.
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