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Schematic of analysis of microlipophagy using the tandem-fluorescent perilipin reporter. N-terminal fusions of mCherry (mCh) and EGFP to PLIN2 label the surface of cytoplasmic LD. Upon uptake of LD into an acidic organelle, such as a lysosome, the EGFP signal is selectively quenched, resulting in punctae only exhibiting mCherry fluorescence. Alexa 647-dextran labeling the lumen of lysosome. Levels of colocalization of mCherry and Alexa 647 indicate levels of LD inside lysosome. EGFP and Alexa 647 exhibited minimal colocalization and were utilized as negative controls. Levels of colocalization of mCherry and EGFP indicate levels of cytoplasmic LD.

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