Representative immunoblotting from immunoprecipitation with GFP-trap beads of the GFP-NuMAWTand GFP-NuMAPARmutin whole-cell extracts of untreated and H2O2-treated (10 M H2O2for 10 min on ice in the dark) cells that were also co-transfected with myc-TDP1. Actin was used as a loading control. Bar charts show the fold change in binding of PARP1, XRCC1, and myc-TDP1 to NuMA in NuMAPARmutrelative to NuMAWT. The bar chart represents data from three biological replicates, with error bars representing the standard error of the mean. Two-sided unpaired Student’s t test was conducted.
