Representative immunoblotting following immunoprecipitation with GFP-trap beads of GFP-NuMAWTand GFP-NuMAPARmutin cells that were either untreated or treated with 10 M H2O2for 10 min on ice in the dark. Bar chart shows the fold change in relative ADP-ribosylation levels (PAR/NuMA) in NuMAPARmutrelative to NuMAWT. The bar chart represents data collected from three biological replicates, with error bars representing the standard error of the mean. Two-sided unpaired Student’s t test was conducted.
