Gel from Scientific Research

RIP-qPCR analysis of Apc mRNA bound to YTHDF1; the immunoblots in (A) show the efficiency of YTHDF1 IP in IgG, empty vector, METTL14 WT, METTL14 I311V, and S399L; the bar graph in (B) shows the Apc levels bound to YTHDF1 in these conditions. Apc mRNA expression is relative to METTL14 WT, and Apc mRNA abundance was normalized to Gapdh in input RNA, n= 3.

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