Quantification of the number of beta-actin puro-PLA puncta in the axons and growth cones of the conditions depicted in (E) and (F) (number of growth cones: ShControl,n= 37; shYthdf1,n= 25; shMettl14,n= 35; shControl + APC,n= 65; shYthdf1+ APC,n= 38; shMettl14+ APC,n= 50; number of axons: ShControl,n= 36; shYthdf1,n= 23; shMettl14,n= 32; shControl + APC,n= 40; shYthdf1+ APC,n= 30; shMettl14+ APC,n= 30). The graphs represent the mean ± SEM in (D), (G), and (B) for beta-actin mRNA density and mean ± SD in (B).∗p< 0.05,∗∗p< 0.01,∗∗∗p< 0.001, and∗∗∗∗p< 0.0001 or ns (not significant) comparing conditions to each other using one-way ANOVA tests (G) and unpaired t test (B and D). See alsoFigures S5,S6, andS7.
