The levels of representative transcripts encoding digestive proteases were scored by RT-qPCR in Dredd mutant intestines with PGRP-LE overexpression in ECs and with or without trametinib treatment. Graph represents log2(FC) in transcript levels relative to untreated Dredd, mex>+ controls. Control values are not shown for clarity but are represented by a blue dotted line. 14 >n> 13 biological replicates (see also Figures S5E and S5F). (A, B, D, F, and G) Dots: individual midguts or biological replicates; horizontal line in the box plot: median; whiskers: minimum and maximum values. Data are from (A) two or (B, D, F, and G) three independent experiments. Asterisks indicate statistically significant differences: (A, B, and D) with mex>+ controls (Mann-Whitney test), (F) between genotypes (Dunn’s multiple comparison test), and (G) between untreated and treated Dredd, mex>PGRP-LE intestines (Dunn’s multiple comparison test). 0.0001 <∗∗∗p≤ 0.001; 0.001 <∗∗p≤ 0.01; 0.01 <∗p≤ 0.05. See also Table S6.
