mRNA-seq and metabolomics analyses were performed in Dredd mutant intestines (Dredd–, mex>+) and Dredd mutant intestines with PGRP-LE overexpression in ECs (Dredd–, mex>PGRP-LE). The schematic shows central carbon metabolism (CCM). Steps in glycolysis, the pentose phosphate pathway (PPP), and tricarboxylic acid (TCA) cycle whose respective transcripts are significantly downregulated in Dredd–, mex>PGRP-LE intestines are marked in blue. Transcript levels are also shown in heatmaps. n= 5 biological replicates. See also Table S1. Dot plots show levels of glycolysis, PPP, and TCA cycle intermediates as well as free amino acids in the midgut, as determined by metabolomics. “Pentose-5P″ corresponds to a pentose phosphate class containing ribose-5-P and ribulose-5-P; pentose phosphates could not be further distinguished. Metabolite levels are normalized to the total protein content of midgut homogenates and are shown relative to Dredd–, mex>+ control midguts. n= 5 biological replicates for Dredd–, mex>+ controls, and n= 4 biological replicates for Dredd–, mex>PGRP-LE. See also Table S2. Dots: biological replicates; horizontal line in the box plot: median; whiskers: minimum and maximum values. Asterisks indicate statistically significant differences from Dredd–, mex>+ (Mann-Whitney test).****p≤ 0.0001; 0.0001 <***p≤ 0.001; 0.001 <**p≤ 0.01; 0.01 <*p≤ 0.05. See also Table S6.
