UMAP from Scientific Research

Schematic of FACS-sorting live nucleated cell (PI-,Ter119-) preparation for post-QC scRNA-seq. Total = 12,390 cells (3 months (mo) [n= 2 combined; 3,876 cells], 3 months [n= 2 combined; 2,929 cells], 12-month WT [n= 2 combined; 3,243 cells], 12-month STING−/−[n= 2 combined; 2,351 cells]). Representative uniform manifold approximation and projections (UMAPs) of ages (3 months = teal; 12 months = coral), genotypes (WT = gold; STING−/−= purple), and neuroimmune-enriched cells clustered and identified as 15 distinct cell types (ImmN: immature neurons; CPEpiC: choroid plexus epithelial cells; OPCs: oligodendrocyte precursor cells; Olig: oligodendrocytes; BAMs: border-associated macrophages; Peri/Epen: pericytes and ependymocytes; EndoC: endothelial cells; Hb-EndoC: hemoglobin-associated endothelial cells; MG: microglia; Mono: monocytes; Neut: neutrophils; B cells, T cells, NKT cells: natural killer T cells.

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