Microscopy from Scientific Research

Activation of auxin responses in root tips upon changes in gravitropic stimuli. Pictures on the left show GFP fluorescence in the root tips of the F1 plants resulting from crossing the stable reporter line DR5:GFP with either C4-expressing plants (35S:C4 lines 8 and 22, T2 generation) or WT (as control), at 5 dpg, before and after 6 h of reorientation. Arrowheads indicate asymmetric distribution of GFP signal in WT (27 out of 49) after 6 h, whereas symmetric expression was detected in C4-expressing plants (27 out of 37) after the same time. Theg-force direction is depicted in the pictures by yellow arrows. In brackets are numbers of roots showing the specific phenotype over the total checked. Scale bar, 10 μm.

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