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Interaction between C4, C4CCLm1, or C4CCLm2 and the plasma-membrane-localized receptor kinases BAM1 or CLV1 by co-IP upon transient co-expression in N. benthamiana leaves. C4- or C4-mutant-GFP band is indicated by an empty arrowhead (in the immunoprecipitate) or by a single asterisk (in the crude protein extract); CLV1- or BAM1-RFP bands are indicated by a filled arrowhead (in the immunoprecipitate) or by a double asterisk (in the crude protein extract). IP, immunoprecipitation; IB, immunoblotting. In both (A) and (B), samples were taken at 2 dpa; the membrane protein IAN9 is used as negative control, and the interaction between C4 and BAM1 is used as positive control. The experiments were performed two times with similar results. One representative replicate is shown here.

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