Bleaching quantification in different lines of 4-week-old SUC:SUL (S–S) Arabidopsis plants expressing C4, C4 Mild, or their respective mutant forms (T2 generation). In the box-and-whiskers graph, each dot represents bleaching values (%) of an individual leaf, and error bars indicate the highest/lowest values. Three leaves per rosette, and a minimum of 10 rosettes per line, were analyzed, when possible. Differences between groups were assessed by applying a Kruskal–Wallis test (p= 4.62E−40, H= 215.43, df = 11), followed by pairwise comparisons between each group and the reference group (S-S/EV line 6) with Mann–Whitney U test (significance 0.0045, after Bonferroni’s correction for multiple comparisons); asterisks represent statistically significant difference: ***p< 0.001; ns, not significant. C4, C4 from TYLCV; C4 Mild, C4 from TYLCV-Mild; S-S, SUC:SUL plants; EV, empty vector-transformed S-S plants.
