Gel from Scientific Research

Open access visualization of Gel, LZY3, C4, RLD3, Co-IP
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Dose-dependent competition between LZY3 and C4 for RLD3 binding by co-IP. Constructs to express RFP-RLD3/RFP-IAN9 and HA-LZY3 with increasing concentrations of C4-GFP were co-transiently transformed into N. benthamiana leaves. The membrane-associated proteins IAN9 (AT1G33970) and LTI6B-HA-TurboID were used as a negative control. After 30 h post -agroinfiltration (hpa), total proteins were extracted and incubated with RFP-Trap agarose. Input and IP proteins were analyzed by western blot with anti-GFP, anti-RFP, and anti-HA antibodies. IP, immunoprecipitation; IB, immunoblotting; BF, bright field. The experiments in(C),(D),(E), and(G)were performed three times with similar results; the experiment in(H)was performed two times with similar results; one representative replicate is shown here. C4, C4 from TYLCV.

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