Bar Plot from Scientific Research

U-2 OS cells stably expressing HT-LC3 were transfected with control or VPS36 siRNAs for 3 days and loaded with membrane-impermeable Alexa Fluor (AF)-488 HaloTag ligand (MIL), followed by MPL. PH, Au, and MAu. Top histogram shows number of HT-LC3 (% of total); bottom histogram shows total number of vesicles/cells. Data are mean +/- SD (n= 3 three independent experiments [10 cells each condition]), two-tailed paired t test,***p< 0.001,**p< 0.01,*p< 0.05; ns, not significant.

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