U-2 OS cells stably expressing HT-LC3 were transfected with control or TfR siRNAs for 3 days and loaded with membrane-impermeable AF-MIL, followed by MPL. PH, Au, and MAu. Top histogram shows the number of HT-LC3 (% of total); bottom histogram shows the total number of vesicles/cells. Data are mean ± SD (n= 3 three independent experiments [10 cells each condition]), two-tailed paired t test,*p< 0.05; ns, not significant. The blot shows the level of TfR with GAPDH as loading control.
