HeLa cells were transfected with control or TfR siRNAs for 3 days, fixed and labeled for endogenous LC3B (green), and processed for super-resolution microscopy. Histogram shows quantification of round (dots) or finger-like LC3B structures in different experiments. Data are mean ± SD (n= 3 independent experiments of minimum 5 cells each condition). Two-tailed paired t test,**p< 0.01. The blot shows the level of TfR, with GAPDH as loading control. Raw images are inFigure S5E. See alsoFigure S5.
