HeLa cells were transfected for 24 h with pEGFP empty vector or pEGFP-MARCH8 treated with DMSO (basal) or Baf for 4 h, processed for immunoblot, and labeled for LC3, MARCH8, GFP, and GAPDH as loading control. Histogram shows LC3-II levels in basal or under Baf treatment, normalized for GAPDH. Data are mean ± SD (n= 4; graphs represent means from four independent experiments. Two-tailed paired t test,*p< 0.05,**p< 0.01. See alsoFigure S4.
