HeLa cells transfected with control or TfR siRNA oligos (mix of oligos 9 and 11) for 5 days and treated with DMSO (basal) or Baf for the last 4 h, processed for immunoblot, and labeled for LC3, TfR, and GAPDH as loading control. Histogram shows relative amount of LC3-II in basal or Baf treatment, normalized for GAPDH. Data are mean ± SD (n= 3; graphs represent means from three independent experiments) two-tailed paired t test,*p< 0.01,*p< 0.05.
