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HeLa cells were transfected with control oligo or siRNA for TfR (oligo 9) for 3 days and transfected in the last 24 h with Myc-empty vector or Myc-TfR-WT (oligo 9-resistant) in combination with the two-half bimolecular fluorescence complementation (BiFC) constructs WIPI2-RAB11A (Split Venus). Histogram shows RAB11-WIPI2 Venus intensity. Data are mean ± SD (n= 3, graphs represent means from three independent experiments). One-way ANOVA with Dunnett’s multiple comparisons test,*p< 0.05; ns, not significant.
#Bar Plot#Microscopy#Error Bars#HeLa Cells#Transfection#Control Oligo#siRNA for TfR#Myc-empty Vector#Myc-TfR-WT#Bimolecular Fluorescence Complementation#WIPI2-RAB11A#Split Venus#RAB11-WIPI2 Venus Intensity#One-way ANOVA#Dunnett’s Multiple Comparisons Test
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