Feeding preference to the 10 mM copper solution in the two-choice CAFE assay. Ecdysteroid biosynthesis is conditionally blocked using tub5-GeneSwitch-GAL4 (tub5-GS-GAL4) and UAS-sad-RNAi in (N) (with RU486 feeding) but not in (O) (without RU486). Note that RU486-containing food was fed 3 days before, but not during the CAFE measurement (M). n= 22, 24, and 23 (J); n= 12, 12, and 11 (K) for tub5-GS-GAL4/+, tub5-GS-GAL4/UAS-sad-RNAi, and +/UAS-sad-RNAi, respectively. Ns:p> 0.05,*p< 0.05,**p< 0.01,***p< 0.001. Kruskal-Wallis test followed by Dunn’s multiple comparison (C, N, and O)
