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[Ca2+]strand [Ca2+]cytinduced by a 10% 405 nm laser (blue bar) in cotyledons of 2BAM4-YC3.6 (n= 6) and NES-YC3.6 lines (n= 5). [Ca2+]ERinduced by sequential 10% 405 nm/20% 488 nm laser (blue bar) in cotyledons of ER-GCaMP6-210 line (n= 6). [Ca2+] in the initial 30 s of HL stimulus was shown in the inset. Curves represent mean ± SEM.
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