Optogenetic silencing of the dFSB in aper0(null) background does not significantly reduce total minutes of sleep relative toper0controls. Top left: sleep in 30-min bins during the second baseline day and first 2 days of silencing from ZT0 to 12 (shown by the green bar). Bottom left: sleep on the third day of silencing from ZT0 to 12 (as indicated by the green bar) and during the first 2 days of recovery. Pink bar indicates the dim red entrainment light from ZT0 to 12. Top right: minutes of sleep from ZT0 to 6. Middle right: minutes of sleep from ZT6 to 12. Bottom right: minutes of sleep for ZT12 to 24. Black asterisks represent significant differences between the experimental animals and theUAScontrol. Blue asterisks represent significant differences between the experimental animals and theGal4control (n= 25–29).*p< 0.05,**p< 0.01,***p< 0.001 by Kruskal-Wallis with Dunn’s multiple comparisons between the experimental andGAL4control and the experimental and theUAScontrol. For line plots showing activity or sleep profiles in 30 min bins distributed over ZT, lines represent means and error bars represent SEM. For box and quartile plots, data are shown representing medians and interquartile ranges, with outliers defined as those whose distance from the median is at least 1.5 times the interquartile range. Related toFigure S3.
