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Representative images and quantifications of neuron-class-specific expression of neuropeptide precursor genes nlp-11 tagged with sl2::gfp::h2b fluorescent reporter alleles in their endogenous genomic loci (z stack max projections, scale bars: 10 m). Sites of expression and divergence are labeled on the fluorescent microscopy images. Bar plots represent proportions of scored animals expressing the corresponding gene in the indicated neuron class. Numbers of scored animals (n) appear below the bar plots. Fisher’s test with Bonferroni correction to multiple comparisons. p< 0.0001, p< 0.001. Corresponding scRNA-seq expression data are shown in dot plots (bottom).

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