Representative images and quantifications of neuron-class-specific expression of the GABA-gated ion channel genelgc-37in head neurons (z stack max projections, scale bars: 10 m). Sites of expression and divergence are labeled. Corresponding scRNA-seq expression data are shown in dot plots. Bar plots represent proportions of scored animals expressinglgc-37in the indicated neuron class. Fisher’s test with Bonferroni correction to multiple comparisons.*p< 0.0001. TheC. briggsaeandC. tropicalisstrains contain a CRISPR-inserted ::t2a::mScarlet3::h2b endogenous reporter allele. For theC. elegansstrain, a fosmid-based construct was used. Cell IDs for RIV, AVD, and AIZ were based on cell position and sequencing data, RMD_LR was confirmed using a srlf-7::gfp array.
