Microscopy from Scientific Research

Phase and Hoechst fluorescence micrographs of L. mexicana parental, FAZ2 null mutant, and FAZ2::mCh add-back after 24 h of induced haptomonad differentiation on gridded glass coverslips. Representative images of adhered cells per grid area are shown for FAZ2 parental, null mutant, and add-back cell lines. Top: phase contrast images. Bottom: Hoechst 33342-stained DNA. Insets show a magnified view of the yellow-dotted squares, illustrating examples of adhered cells per cell line. Scale bars, 100 μm.

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