Microscopy from Scientific Research

Autophagic flux in mouse primary cortical neurons from mRFP-GFP-LC3 (tfLC3) transgenic mice. Representative confocal z-stack images (right panel) and total number of mRFP + GFP dots (autophagosomes) and mRFP-only dots (autolysosomes). In total, n= 4 independent experiments, ≥30 cells analyzed per condition per experiment; two-tailed paired t test.**p< 0.01,***p< 0.001 vs. control cells. Scale bar, 10 and 2 μm (enlarged images).

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