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Schematic of monosynaptic anterograde tracing from fasting-TRAPed neurons in the PBNdl. Cre-dependent GFP-2A-synaptophysin-mRuby virus injected into the PBN of Fos2A-iCreER mice. Right: representative confocal image GFP and mRuby expression in PBNdl neurons. Lower: Protocol showing targeted recombination in active populations (TRAP) induction by 4-OHT injection after 18 h of fasting. Scale bar: 50 μm.
#Workflow#Illustration#Microscopy#Monosynaptic Anterograde Tracing#Fasting-TRAPed Neurons#PBNdl#Cre-dependent GFP-2A-synaptophysin-mRuby Virus#PBN#Fos2A-iCreER Mice#Confocal Image#GFP#mRuby Expression#Targeted Recombination in Active Populations#TRAP#4-OHT Injection#Fasting#Scale Bar
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