HEK293 cells were transiently co-transfected with V5-HDAC9 or V5-empty control vector and FLAG-tagged full-length NLRP3, NLRC4, or AIM2. Shown are representative immunoblots depicting the binding of HDAC9 to NLRP3, but not NLRC4, and AIM2.n= 4 independent experiments. See also Figures S5O–S5Q.