Gel from Scientific Research

Schematic of an in vitroliposome assays using commercially available di-oleoyl BMP that was used to assess PLA2G15 activity (top). The expected products from PLA2G15 degradation of di-oleoyl BMP are oleic acid and lysophosphatidylglycerol (LPG). The PLA2G15 inhibitor DFP was used as negative control. In the present solvent system (seemethod details), one of the products, oleic acid, migrated onto the plate and was detected after release from BMP (n= 4 technical replicates/group).

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