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Schematic diagram showing the determination of neuronal activation in the brain by c-Fos staining in WT mice. WT mice were injected intratracheally (i.t.) with saline (sham), 1 x 105 CFU E. coli (mild), 1 x 106 CFU E. coli (moderate), 3 x 106 CFU E. coli (severe). c-Fos expression across different regions of the brain was assessed 24 h post E. coli infection.

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