Proportion of escape mutations seen in over 1,000 strains in Global Initiative on Sharing All Influenza Data (GISAID), as predicted by EVEscape (with and without SARS-CoV-2 sequences), high-throughput DMS experiments across 20 studies (Table S5), or randomly selected RBD mutations. Predicted escape mutations were defined as nonsynonymous, single-nucleotide RBD mutations in the top 5% of escape scores for computational and experimental methods (top 1% and 10% inFigure S4). Points indicate updates to the set of escape mutations based on the availability of a more recent DMS data (blue) or model (red, which includes SARS-CoV-2 sequences available prior to that date). Predictions for the full spike protein (computational) and for each independent experiment and model are provided inFigure S4.
