Oil red O staining of liver large lobes from the mice. Scale bar: 20 μm. Lp-EV, empty vector; Lp-Lep, Lp-MSH, and Lp-BDNF, engineered Lp strains secreting leptin, α-MSH, and BDNF, representatively. Lp-Mix, mixed regimen of all three engineered strains. n= 5 mice unless otherwise stated. Individual data points are indicated in color circles. Error bars indicate the standard error. Two-way repeated measures ANOVA followed by Dunnett’s post hoc tests was performed to determine statistical significance of the data to the EV control in (B) and (E). One-way ANOVA followed by Dunnett’s post hoc tests was performed to analyze the rest of the data. *p< 0.05; **p< 0.01; ***p< 0.001. See also Figures S6 and S7.
