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C to T substitution frequencies at the ends of reads for all sequences (black). The orange line represents selecting sequences carrying C to T substitutions at their 5' ends, so the C to T substitution frequency is 100% at the 5' ends and 34% at the 3' ends. The blue line represents selecting sequences carrying C to T substitutions at their 3' ends, where the C to T substitution frequency is 100% at the 3' end and 52% at the 5' end.
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