Percentage of time in each brain state following vehicle or GH infusion in DBH-Cre mice bilaterally injected with sgControl or sgGHR virus (n= 11 mice per group). sgControl, p(NREM) = 0.018, p(wake) = 0.004, p(REM) = 0.0008. sgGHR, p(NREM) = 0.88, p(wake) = 0.74, p(REM) = 0.10 (two-way repeated measures ANOVA, main effect of treatment). sgControl-wake: p(1.5 h) = 0.021, p(2 h) = 0.004, p(2.5 h) = 0.0003, p(3 h) = 0.009; sgControl-REM: p(2 h) = 0.005, p(2.5 h) = 0.002, p(3 h) = 0.006, p(3.5 h) = 0.008, p(4 h) < 0.0001 (Bonferroni’s multiple comparisons test). Error bar, ± SEM. Bar and error bar, mean ± SEM.∗p< 0.05;∗∗p< 0.01;∗∗∗p< 0.001;∗∗∗∗p< 0.0001; ns, not significant. See also Figure S6.
