Gel from Scientific Research

Proteasomal subunit activity in neurons overexpressing mScarlet (control), PSMB5 (PSMB5OE), or PSMB8 (PSMB8OE). One lane without the activity probe was used as a negative control (labeled with (–)). Hybridoma cells, either untreated (veh.) or treated with the proteasome inhibitor epoxomicin, (ep.) served as positive and negative controls, respectively. Immunoblots (IBs) of PSMB5, PSMB8, and PSMA2 (proteasome loading control) corresponding to the activity gels across all conditions are shown. A red arrowhead marks the activity band of the overexpression constructs. In the PSMB5 IB, a black arrowhead indicates endogenous PSMB5, while blue arrowheads indicate size shift of endogenous/exogenous PSMB5 due to activity-based probe binding. In the PSMB8 IB, blue arrowheads denote a size shift of exogenous PSMB8 due to activity-based probe binding. In total 4 replicates were performed.

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