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IVT-U, IVT- Psi, and IVT-m1Psi were digested with RNase T2 (0.5 nM), PLD3 (25 nM), or a combination of RNase T2 (0.5 nM) and PLD3 (25 nM) for 1 h and the release of 2',3'-cGMP was analyzed by LC-MS. Mean ± SEM of n= 3 independent experiments.

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