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A 2,500 nt IVT (1 g) was digested with RNase T2 (3.5 M) or RNase 1 (3 nM). Representative LC-UV and LC-MS chromatograms of the two digests are shown (only the elution window from 1230 min is shown). Annotated are fragment sequences with their corresponding masses (i.e.,m/zvalues) as identified by LC-MS. The chromatograms are representative for two (RNase T2) or three (RNase 1) independent experiments.
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