Gel from Scientific Research

Strep-tag pull-down experiments were conducted to assess the binding compatibilities of NAbs across different groups. HA-tagged Fabs containing Strep tags were bound to Streptactin resin as bait. GFP-tagged M-HBsAg was added to mediate the binding between compatible NAbs. Myc-tagged Fabs or scFv served as prey. Proteins were detected using western blotting (for HA or Myc tags) or in-gel fluorescence imaging for GFP (M-HBsAg).

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