Microscopy from Scientific Research

Virtual staining of iNeuron cells recovers the contrast needed for soma segmentation and neurite tracing. Cell membranes (magenta) and nuclei (green) staining are preprocessed to filter debris in cell culture and normalize contrast. Virtually stained nuclei and cell bodies are shown in blue and orange. The cyan arrows in the preprocessed fluorescence image point to white pixels where cell bodies and nuclei overlap. Soma segmentation is shown in colour-filled labels and neurite tracing is shown in white lines. Scale bars, 100 m.

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