High-magnification images showing HT-1080 ANLN/Ect2 (WT) cells in the process of extravasating out of the CAM vasculature. The bottom panels show GFP-anillin only. In, intravascular; Ex, extravasating; Out, extravasated cells. Exposure in o and p is increased to show cytoplasmic anillin. Scale bars, 20 m.