Microscopy from Scientific Research

Increasing confinement elevates RhoA activity and polarization as measured by FLIM–FRET of a RhoA2G biosensor. Representative cells in channels of the prescribed cross-sectional area. Greyscale images of donor intensity and pseudocolour images showing the subcellular distribution of activated RhoA were prepared with SymPhoTime 64 from single confocal sections acquired with the PicoQuant FLIM system. Scale bars, 10 µm.

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