Scatter Plot from Scientific Research

Paracetamol synthesis by one-pot Lossen rearrangement and bacterial whole-cell synthesis. Strain E. coli _p350 expresses ABH60 and strain E. coli _p354 expresses PANAT K211G. Ratios refer to E. coli _p354 and E. coli _p350 in biotransformations (1:1, OD 600 26; 1:5, OD 600 16; 1:100 and 1:200, OD 600 12.5). a Final cell density OD 600 25. OD refers to the final optical density at 600 nm. b Cells induced with 0.5% l-arabinose. Reaction conditions: (i) substrate (0.5 mM), aqueous potassium phosphate (200 mM, pH 8.0), 50 °C, 48 h; (ii) E. coli _p354 and E. coli _p350 (1:10; OD 600 20). Biotransformations were analysed by 1 H NMR relative to an internal standard of trimethoxybenzene or by HPLC relative to an internal standard of caffeine. All data are presented as mean values ± s.d. of three biological replicates.

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