Gel from Scientific Research

Denaturing PAGE, which disrupts the duplex formed by HMSD, is used to detect which systems have formed covalent bonds. The monomers are converted to dimers after hybridization of M 1 an N 1 (‘M-alkyl + N-aza’) but not if binding is inhibited by the presence of lock strands (‘blocked M-alkyl + N-aza’). The action of a template T 11 (at a ratio of 1:10 with M 1 L ) during 24 h restores dimerization. Top: the labels indicate the initial concentrations of M 1 L and N 1 .

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