Gel from Scientific Research

A non-denaturing PAGE analysis of reaction products after mixing either A 1 , B 1 and C 1 ; A 2 , B 2 and C 2 ; or both, with various combinations of templates. The formation of intended products is minimal in the absence of the relevant templates but visible when the templates are present. The bands, including intermediates and unintended products, can be identified by comparing the fluorescence in three channels and migration speed to controls (Supplementary Results 5 and Supplementary Figs. 33 – 35 ).

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