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TARGET-seq analysis of n = 1,903 cells from four chr. 21amp donors with allelic resolution of mutant JAK2 / TP53 and chr. 21amp event in single cells enables inference of clonal hierarchy. In total, 107 cells had no genomic aberration, while 179 cells were mutated for JAK2V617F alone. Further, 162 cells were double JAK2 and TP53 mutant, with no evidence of chr. 21amp, while 1,455 cells carried all three genomic aberrations.

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