MA plots showing chromosome-located ( e ) or pINV-located ( f ) Shigella gene mutant relative abundances upon infection of enteroids, as quantified by TraDIS. Shown is the log 2 FC in the output library on the y axis and the log 2 CPM (average log 2 -transformed counts per million) on the x axis as detailed in d ; each dot represents a gene. CDS, coding sequence; Pseudo, yellow, pseudogene.