Workflow from Scientific Research

A schematic of a typical entrapment method. The target database is augmented with entrapment sequences, and the augmented database is used by the search tool to produce a ranked list of peptides. In this example, the entrapment is done via the common approach of database expansion and the tool is using decoys to control the FDR, but other methods can be used. The target/entrapment labels are hidden from the search engine but are revealed to the entrapment method, allowing it to provide an estimated FDP. Note that some entrapment estimation methods require additional inputs besides the count of the number of original and entrapment targets.

DOI