Representative images of basal (Krt5+) and spinous (Krt13+) cells with an inset showing Net1 mRNA localized to the basal tips of Krt13+ cells (yellow arrowhead). Line and bar graph data are measurements from individual tissue regions (ROIs) with mean ± SEM; n = 32 ( d , e ) or 36 ( g ) ROIs from N = 4 mice. Data from individual mice are represented with different colors. ** P ≤ 0.01, **** P ≤ 0.0001, ns non-significant by Brown–Forsythe and Welch ANOVA followed by Dunnett’s T3 multiple comparison test ( e ), or unpaired two-tailed Student’s t -test ( g ). Source data are provided as a Source Data file.