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As described in Fig. 3a , tumors derived from Black Braf CA/+ ; Pten Δ/+ were serially passaged by transplantation into NSG (immune deficient) mice. Tumors from one parental Black Braf CA/+ ; Pten Δ/+ genotype mouse and two serially transplanted NSG mice (derived from a single tumor) were subjected to scRNA-seq, analyzed separately, and the neural crest-derived cells were sub-clustered accordingly (parental black Braf CA/+ ; Pten Δ/+ tumor: 2686 cells; serially transplanted NSG tumor #1 and #2: 10,662 and 10,965 cells respectively).
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