The proportion of stationary surface associated cells after 2 min correlates with subsequent bacterial attachment (fluorescence intensity; AU, arbitrary units) after 4 h incubation. Data shown are mean SD. n = 739, 813 and 809 single bacterial cells respectively tracked on flat, pro-attachment (TopoUnit 697) and anti-attachment (TopoUnit 881) surfaces. For Fig. 5d, e, the source data are provided as a Source Data file.